Thequbit4FlurometeristhelatestversionofthepopularQubitfluorometerdesignedtoaccuratelymeasureDNA,RNA,andproteinquantity,andnowalsoRNAintegrityandquality,usingthehighlysensitiveQubitassays.TheQubit4Fluorometerwasre-engineeredtoruntheQubitRNAIQ(integrity&quality)assay.TheQubit4FluorometerandRNAIQAssayKitworktogethertoaccuratelydistinguishintactfromdegradedRNAinjusttwoeasysteps.
ForallQubitassays,theconcentrationorqualityofthetargetmoleculeinthesampleisreportedbyafluorescentdyethatemitsasignalonlywhenboundtothetarget,whichminimizestheeffectsofcontaminantsontheresult.Theeasy-to-usetouch-screenmenusmakeiteasytoselectandruntheassaysyouneed,withresultsdisplayedinjustafewseconds.
KeyfeaturesoftheQubit4Fluorometerinclude:
•FastandhighlyaccuratequantitationofDNA,RNA,andproteininlessthanthreesecondspersample
•MeasuresintactRNAinlessthan5secondspersample
•Highlevelsofaccuracyusingonly1–20μLofsample,evenwithverydilutesamples
•On-boardReagentCalculatorquicklygeneratesQubitworkingsolutionpreparationinstructions
•Storesresultsfromupto1000samples
•Large5.7-inch,state-of-the-artcolortouchscreenforeasyworkflownavigation
•Graphicaldisplayindicateswhensamplesareintheextendedrangeoroutofrange
•Smallfootprintsavesspaceonyourbench
•ExportsdatatoaUSBdriveordirectlytoyourcomputerviaaUSBcable
•ABIlitytopersonalizeyourQubitfluorometerwiththeassaysyourunmost,addnewassays,orevencreateyourownassayswiththeMyQubitsoftwareandwebtool
•LanguageofyourchoiceincludingEnglish,French,Spanish,Italian,German,simplifiedChinese,andJapanese
TheQubit4FlurometercanalsobeusedtodirectlymeasurethefluorescenceofsamplesusingFluorometermode.Additionally,IonSphereParticlequalitycanbeevaluatedontheQubit4FluorometerusingtheIonSphereQualityControlKitpriortoperformingasequencingrunontheIonPGMsequencer.
EnablesmoreaccurateresultsthanwithUVabsorbanceatlowconcentrations
TheQubit4FluorometerisfarmoresensitivethanUVabsorbance,whichmeasuresanythingabsorbingat260nm,includingDNA,RNA,protein,freenucleotides,orexcesssalts.Moreover,UVspectrophotometryisoftennotsensitiveenoughtoaccuratelymeasurelowconcentrationsofDNAandRNA.WiththeQubitfluorometer,yourresearchisenhancedbymoreaccuratemeasurementsbecausethedyesintheQubitassaykitsfluoresceonlywhenboundtotheselectedmolecule(DNA,RNAorprotein)inyoursample.Thisallowsyoutoavoidrepeatingworkduetoinaccuratemeasurements.
Fast,simple,andaccuratemeasurementofRNAquality
TheQubitRNAIQassayprovidesafast,simplemethodtocheckwhetheranRNAsamplehasdegraded.Theassayutilizestwouniquedyes:onethatbindstolarge,intactRNA,andanotherthatselectivelybindstosmall,degradedRNA.TogethertheyenableyoutoquicklyassessthequalityandintegrityofanRNAsample.Touse,simplyaddyoursamplestotheRNAIQworkingsolution,thenmeasureontheQubit4Fluorometer.
Savesamplesandbenchspace
ThethoughtfullydesignedQubit4Fluorometerrequiressamplesofonly1–20µL.Itisintendedforuseatroomtemperatureandoccupiesonlyasmallamountofbenchspace.TheQubit4Fluorometerfeaturesadvancedopticsanddataanalysisalgorithms,andcomesequippedwithaUSBthumbdriveandcablefordatatransfertoExcel™softwareandforsoftwaredownloads,aswellasauniversalpowersupply,fourplugadapters,andelectromagneticCEcertification.
Singlesamplemeasurementsareabreeze
Thisuser-friendlyfluorometeriscombinedwithquickandsimpleassayprocedures.Calculationsandsettingsareautomaticallyperformedbytheinstrument.TheQubitassaysforusewiththeQubit4Fluorometerareallperformedusingthesamegeneralprotocol,whichusesasimplemix-and-readformatwithincubationtimesofonlytwominutesfortheDNAandRNAassays.500Lthin-walledPCRtubes(Cat.No.Q32856)arerequired.
Systemspecificationsinclude:
Instrumentdimensions: 5.4"(w)x10"(l)x2.2"(h)(13.6cmx25cmx5.5cm)
Weight: 743g
Dynamicrange: 5ordersofmagnitude
Processingtime: ≤seconds/sample
Lightsources: blueLED(max~470nm),redLED(max~635nm)
Excitationfilters: blue(430–495nm),red(600–645nm)
Emissionfilters: green(510–580nm),red(665–720nm)
Detectors: photodiodes,measurementcapabilityfrom300–1,000nm
Warm-uptime: <35seconds
USBthumbdrive: 4GB
赛默飞世尔科技公司(Thermo Fisher Scientific)赛默飞世尔科技是全球科学服务领域的领导者(纽约证交所代码:TMO),致力于帮助客户使世界更健康,更清洁,更安全。公司年销售额170亿美元,在50个国家拥有员工约50,000人。主要客户类型包括医药和生物公司,医院和临床诊断实验室,大学、科研院所和政府机构,以及环境与工业过程控制装备制造商等。
公司借助于Thermo Scientific、Life Technologies、Fisher Scientific和Unity™ Lab Services四个首要品牌,帮助客户解决在分析化学领域从常规的测试到复杂的研发项目中所遇到的各种挑战。Thermo Scientific能够为客户提供一整套包括高端分析仪器、实验室装备、软件、服务、耗材和试剂在内的实验室综合解决方案。Fisher Scientific为卫生保健,科学研究,以及安全和教育领域的客户提供一系列的实验室装备、化学药品以及其他用品和服务。Thermo Fisher Scientific将努力为客户提供最为便捷的采购方案,为科研的飞速发展不断地改进工艺技术,提升客户价值,帮助股东提高收益,为员工创造良好的发展空间。